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rbsB [2018-05-04 12:19:07]
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rbsB [2018-05-04 12:19:07]

ribose ABC transporter (binding protein)
locus
BSU35960
pI
6.14
mw
32.07 kDa
protein length
305 aa Sequence Blast
gene length
915 bp Sequence Blast
function
ribose uptake
product
ribose ABC transporter (binding protein)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.1|Uptake of carbon sources]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.4|Utilization of ribose]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,706,145 → 3,707,062

    The protein

    Protein family

  • bacterial solute-binding protein 2 family (according to Swiss-Prot)

    • Structure

  • [PDB|2GX6] (from ''Escherichia coli k12 mutant'', 54% identity, 71% similarity)

    • [SW|Localization]

  • extracellular (signal peptide), lipid modification as retention signal [Pubmed|18957862]
  • attached to the cell membrane (via [protein|F1258E31151E137A850811B0C851D473CEFFD43B|RbsC]-[protein|F0E6F191FAF8F279462B09BF1FE4C6C752A57803|RbsD]) [Pubmed|10092453]

    • Expression and Regulation

    Operons

    genes
    [gene|77AEAC0F06B4D5F062D4A2740794B7BFDDA31096|rbsR]-[gene|C229BD2C00B169CF4A99482E65B3F749AD9D51D8|rbsK]-[gene|F0E6F191FAF8F279462B09BF1FE4C6C752A57803|rbsD]-[gene|AB4A686F62550F90F5BD8D27DAADC9C33F1DA1DB|rbsA]-[gene|F1258E31151E137A850811B0C851D473CEFFD43B|rbsC]-[gene|1D67888E79B09956F9258F4068546D8C67103549|rbsB]
    description
    [Pubmed|7921236]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7511775], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|7921236], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: activation, [Pubmed|7592460], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

    • regulation

  • expressed upon transition into the stationary phase ([protein|search|AbrB]) [Pubmed|7592460]
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    Biological materials

    Mutant

  • BKE35960 (Δ[gene|1D67888E79B09956F9258F4068546D8C67103549|rbsB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAACCCTCCTAAGC, downstream forward: _UP4_TAATTGTCTGATGTTTAGGA
  • BKK35960 (Δ[gene|1D67888E79B09956F9258F4068546D8C67103549|rbsB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAACCCTCCTAAGC, downstream forward: _UP4_TAATTGTCTGATGTTTAGGA

    • References

  • 10092453,16872404,7511775,7592460,18957862,7921236